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BD Phoenix™ Panels

Timely and accurate detection of known and emerging antimicrobial resistance.1-6

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Overview

BD Phoenix™ panels, when used with the BD Phoenix M50, provide timely and accurate identification and susceptibility results for most clinically significant aerobic and facultative anaerobic Gram-negative and Gram-positive bacteria, as well as identification of yeast and yeast-like organisms.1-6  

 

BD Phoenix™ panels require no refrigeration, are leak resistant, and are available in a variety of formats including identification-only, susceptibility-only, and combination of identification and susceptibility panels, for use with the BD Phoenix™ M50 Automated Microbiology System.

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Microorganism identification with BD Phoenix™ panels

The BD Phoenix™ identification-only panels as well as combination identification and antimicrobial susceptibility panels use 51 wells for biochemical tests to identify most clinically significant bacteria and yeast.

<p><b>Susceptibility testing with BD Phoenix™ panels</b> </p>

Susceptibility testing with BD Phoenix™ panels

The standard antimicrobial susceptibility testing-only panels and combination ID/AST panels use 85 wells for antimicrobial dilutions. The BD Phoenix™ Emerge™ panel leverages 136 wells for susceptibility testing. Detectable antimicrobial resistance markers include* methicillin-resistant Staphylococcus aureus (MRSA), mecA-mediated resistance, vancomycin-resistant S. aureus (VRSA) and Enterococcus spp. (VRE), extended-spectrum β-lactamase (ESBL)-producing organism, high-level aminoglycoside resistant (HLAR) Enterococcus and Streptococcus spp., carbapenemase producing organism (CPO), and inducible clindamycin resistance (iMLSb).

 

*Non-exhaustive list. Depending on BD Phoenix™ panel type and organism identification.

<p><b>BD Phoenix™ Emerge™ Panels</b></p>

BD Phoenix™ Emerge™ Panels

The BD Phoenix™ Emerge™ Gram-negative and Gram-positive panels offer 136 wells for susceptibility testing, allowing for additional antimicrobial dilutions or additional drugs, to provide the foundation for the detection of emerging resistance.

<p><b>Detecting carbapenamase-producing organisms (CPOs)</b></p>

Detecting carbapenamase-producing organisms (CPOs)

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Microorganism identification

  • The BD Phoenix™ system offers identification-only panels and combination ID/AST panels, using 51 wells for identification substrates.
  • The instrument can provide rapid identification of most clinically significant Gram-negative and Gram- positive bacteria, as well as yeast.

Susceptibility testing

  • The BD Phoenix™ system offers 85 wells for antimicrobial dilutions on standard AST-only panels and combination ID/AST panels, and 136 wells on the BD Phoenix™ Emerge™ panel.
  • The instrument can provide rapid susceptibility results for most clinically significant Gram-negative and Gram-positive bacteria.
  • The BD Phoenix™ system panels test for several resistance markers*such as**:
    • Gram-positive bacteria
      • HLAR—High Level Aminoglycoside Resistant Enterococcus
      • iMLSb—Inducible Clindamycin Resistance
      • MRSA—based on Oxacillin Interpretation with Staphylococcus species
      • mecA - detection in mecA-mediated resistance Staphylococcus Aureus
      • BL-Staphylococcus ß-Lactamase (Nitrocefin-based test)
      • VRSA—Vancomycin-Resistant Staphylococcus Aureus
      • VRE—based on Vancomycin interpretation
    • Gram-negative bacteria
      • CPO—Carbapenemase Producing Organism
      • ESBL—offered on Gram-negative panels and requires no additional disc diffusion or E-Test confirmation

* Depending on BD Phoenix™ panel type and organism identification.

Detecting CPOs

  • The BD Phoenix™ CPO detect test, available on certain BD Phoenix™ Gram-negative panels, provides information, including Ambler classification, to help guide clinicians in their treatment decisions.
  • This test is available in two-panel configurations that allow microbiology labs to test for CPOs as part of routine susceptibility testing on BD Phoenix™ panels:
    • 2-well configuration—provides detection of Carbapenemase-producing organisms in Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii
    • 9-well configuration—provides detection of Carbapenemase-producing organisms and Ambler classification (A, B or D) in Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii
  • The BD Phoenix™ CPO detect test is currently the only phenotypic test on an automated AST system that provides CPO detection and Ambler classification.
  • On average, the BD Phoenix™ CPO detect test detects and classifies CPOs from isolated colonies within 6 to 11 hours**.

**BD internal studies—data on file

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Resources
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Reference
  1. BD Phoenix™ M50 Automated Microbiology System User's Manual (500008930).
  2. Carey RB et al. Failure of Automated Systems to Detect Vancomycin-Resistant Staphylococcus aureus. Abstract presented at the Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), Washington, DC, 2004.
  3. Tenover FC et al. Emerg Infect Dis. 2006;12(8):1209–13. 
  4. Kulah C et al. BMC Infect Dis. 2009;9:30.
  5. Mittman SA et al. J Clin Microbiol. 2009;47(11):3557–61.
  6. Woodford N et al. J Clin Microbiol. 2010;48(8):2999–3002.
  7. BD™ Bruker MALDI Biotyper CA System User Manual (8603291).
  8. Saffert RT et al. J Clin Microbiol. 2011;49(3):887–92. 
  9. Knabl L et al. Lett Appl Microbiol. 2021;73(1):2–8.
  10. Watanabe N et al. J Infect Chemother. 2022;28(4):563–8.
  11. Accelerate PhenoTest BC kit Instructions for Use (LBL000172).